Cladribine triphosphate is the active compound of the anti-cancer and multiple sclerosis drug Mavenclad (cladribine). Biosynthesis of such non-natural deoxyribonucleotides is challenging but important in order to study the pharmaceutical modes of action. In this study. we developed a novel one-pot enzyme cascade for the biosynthesis of cladribine triphosphate. https://lockdownsecuritycanada.shop/product-category/resistor-kit/